Department of Protein Biochemistry and Proteomics

The main focus of the workplace is mass spectrometry in protein analysis. Research staff have access to equipment for protein and peptide separation supplemented with mass spectrometers with MALDI and ESI ionization. Scientists use common methods for sample preparation and purification, routine protein identification (peptide mapping and peptide sequencing), relative and absolute quantification, and analysis of post-translational modifications (glycosylation, phosphorylation). Their range of activities is wide: from analysis of one selected protein to proteome mapping. Intact cell mass spectrometry and spore help to identify microorganisms. Enzymes, especially of plant and microbial origin, such as dehydrogenases, oxidases and proteases, are also studied at the department. These enzymes are obtained by isolation from their natural sources (tissues, cells) or via recombinant expression of the respective genes in host microorganisms. Their studies include the description of molecular properties, the identification of typical substrates and inhibitors, eventually crystallization and structural analysis. Researchers also look for practical use of enzymes in food analysis by the construction of biosensors or the immobilization of enzymes on solid carriers. Research into metabolic pathways for biosynthesis and biodegradation of certain metabolites (e.g. plant hormones) is also one of the research directions of this department.

http://proteomika.cr-hana.upol.cz/index_en.php

prof. Mgr. Marek Šebela, Dr.

Head of department,Leader of research programme, Member of Scientific Board, Faculty of Science, Palacky University
+420 585 634 927, +420 585 634 922, marek.sebela@upol.cz

He majored in Biochemistry at the Faculty of Science, Masaryk University in Brno, Czech Republic, where he also later completed his doctoral study in the same discipline. He moved to Palacký University Olomouc to develop his research and academic career. He became a Professor of Biochemistry at Palacký University Olomouc in 2007 and worked as the Head of the Department of Biochemistry, Faculty of Science during 2006-2012. In the meantime, he completed several short-term research stays abroad (Germany, Denmark, France, Japan). Between 2014 - 2018, he was the Deputy Director of CRH, where he has been leading the Department of Protein Biochemistry and Proteomics since 2010. He focuses on chemistry and biochemistry of proteins. His research is mainly devoted to redox and proteolytic enzymes, protein modifications (namely glycosylations) and MALDI mass spectrometry in proteomics. In addition to research, teaching and educational activities, he is an active member of three scientific societies and participates in organizing international scientific conferences such as Informal Meeting on Mass Spectrometry or FEBS Congress. He received two national scientific awards. He is a member of the editorial board of Journal of Proteomics.

Education:

  • 1994 - graduated in Biochemistry from the Faculty of Science, Masaryk University, Brno, CZ
  • 1997 - receiving Ph.D. degree in Biochemistry at the Faculty of Science, Masaryk University, Brno, CZ
  • 2002 - habilitation in Biochemistry at the Faculty of Science, Masaryk University, Brno, CZ
  • 2007 - successful passing the professorship nomination procedure in Biochemistry at Masaryk University in Brno, CZ

Employment:

  • 1994 - 1996 - research assistant at the Faculty of Science, Palacky University in Olomouc, CZ
  • 1996 - 2002 - assistant professor at the Faculty of Science, Palacky University in Olomouc, CZ
  • 2002 - 2007 - associate professor at the Faculty of Science, Palacky University in Olomouc, CZ
  • 2006 - 2012 - Head of the Department of Biochemistry, Faculty of Science, Palacky University, CZ
  • since 2007 - Full Professor in Biochemistry at the Faculty of Science, Palacky University, CZ
  • since 2010 - Head of the Department of Protein Biochemistry and Proteomics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, CZ
  • 2014 - 2018 - Deputy Director, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, CZ

Research orientation:

  • enzymology; he especially deals with enzymes from the metabolism of polyamines
  • protein biochemistry and proteomics; he is interested in proteolytic enzymes and glycoproteins
  • he published more than 130 scientific papers (150 entries at the Web of Science; 1800 citations, h-index 21); more than 200 conference presentations
  • he was awarded the Junior Scientist Award by the Czech Learned Society (2005)
  • he was bestowed with the Hanus and Sedmera Prize by the Czech J.M. Marci Spectroscopic Society for his work focused on elucidation of molecular structure using mass spectrometry (2010)

International experience:

  • 1995 (1 month): University of Bayreuth, Germany; Ph.D. study stay
  • 1996 (2 months): University of Bayreuth, Germany; Ph.D. study stay
  • 1997 (1 month): Carlsberg Laboratory, Denmark; Ph.D. study stay
  • 2000 (1 month): universities in Yamaguchi and Osaka, Japan; research stay
  • 2002-2006 (3 months in total): Max Planck Institute for Molecular Cell Biology and Genetics, Germany; research stays within a joint research project
  • 2006 (1 month): AgroParisTech, France; sabbatical leave
  • many short stays (1-2 weeks): Bayreuth, Cagliari, Eger, Lyngby, Rome, Vienna, Arlington/Texas
  • Erasmus teaching stays in Lyngby, Grignon and Versailles

Membership:

  • Czech Society for Biochemistry and Molecular Biology (ČSBMB); Executive Committee member
  • Czech Proteomic Society (an affiliate to ČSBMB); Executive Committee member
  • Czech Society for Structural Biology; Executive Committee member
  • Czech Society for Mass Spectrometry; member
  • European Proteomics Association; national society representative
  • member of the Editorial Board of Journal of Proteomics
  • member of the Board of Central and Eastern European Proteomic Conferences

Grants and projects:

  • principal investigator (8) or team member (25) in national (MEYS, GAČR) and international (BMBF/Germany, OeAD/Austria, JSPS/Japan) projects
  • principal coordinator (1) or team member (14) of national educational projects (MYES)